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This study was performed to analyze the purity of technical grade α-naphthylamine, to establish optimal analytical condition of α-naphthylamine in urine and to determine the urine sample of workers exposed to α-naphthylamine. The purity of technical grade α-naphthylamine were 96.5±2.38%, 94.1±0.97%, 97.0±0.02% by gas chromatography-mass selective detector. To analyze α-naphthylamine in urine, high performance liquid chromatography-electrochemical detector and gas chromatography-electron capture detector operating conditions have been optimized by preliminary experiment. In high performance liquid chromatography-electrochemical detector, the mobile phase was consisted of acetonitrile(35%) and water(65%), and the flow rate was maintained at 1.0㎖ per minute. Optimal detective condition was 9.0V(10nA/V) of electrochemical detector. The recovery of sep-pak treatment method was highly estimated as pretreatment of α-naphthylamine in urine. The free amine was isolated by Bas chromatography-electron capture detector after basic hydrosis, sep-pak treatment, toluene elution and HFBA(heptafluoro-butyric anhydride) derivatization of urine. The recovery of α-naphthylamine in urine was 98.73±3.29% by gas chromatography-electron capture detector. The sensitivity was more higher than that of high performance liquid chromatography-electrochemical detector. Urinary α-naphthylamine was detected in only one worker among nine workers. The level of α -naphthylamine in urine was 6.42 ㎎/㎖.
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